WESTERN BLOTTING
Amersham™ ECL start
Why use a more sensitive, more expensive ECL reagent if you don’t need it?
Applications: Features
• Routine protein detection • No rush - long lasting 3 hour signal duration
• High expressed proteins • Produces low background on film or CCD
• e.g. house keeping proteins • Working solution is stable for 5 days! 5x longer than others!
• Tagged proteins • 18 month shelf life! 6-9 months longer than others!
Cat # Descripción Presentación
85-662 ECL start Reagent, 200ml Kit 2000cm2
85-663 ECL start Reagent, 400ml Kit 4000cm2
Amersham™ ECL Prime
• A highly sensitive chemiluminescent detection reagent
• Intense and long lasting signal enabling large experimental series
• Detect both high and low proteins on the same blot after a single exposure
• High to low protein levels. LOD: Low picograms <10pg
Cat # Descripción Presentación
Amersham™ ECL Select
• The most sensitive reagent -detects the smallest changes in your protein of interest
• Exceptional signal intensity resulting in brighter, clearer bands at low protein levels
• Delivers high sensitivity even when using highly diluted primary and secondary antibodies
• Medium to very low proteins levels. LOD: Low picograms <5pg
Cat # Descripción Presentación
84-836 ECL Select Reagent, 100ml Kit 1000cm2
Amersham™ Rainbow Molecular Weight Markers
• Faster and simpler identification of proteins on SDS polyacrylamide gels.
• Sharp, intense bands with ample band spacing for accuracy.
• Bright, distinctive colors allow confirmation of transfer and orientation.
• Asses transfer efficiency and MW of blotted proteins without staining.
Cat # Descripción Presentación
83-650 Full Range MW Marker, 2mg/ml 50 Applications
83-651 High Range MW Marker, 1.5mg/ml 50 Applications
83-652 Low Range MW Marker, 1mg/ml 50 Applications
Membranas en Rollo, Hojas & Sandwiches
Amersham Protran™ Membranas de Nitrocelulosa
• For chemiluminescence detection of high abundant proteins.
• Use 0.2 for small proteins and peptides < Mr 20,000
• Use 0.45 for proteins in a wide range of molecular weights.
Amersham Hybond™ P PVDF Membranas
• For chemiluminescence detection of low abundant proteins.
• Use 0.2 for small proteins and peptides < Mr 20,000
• Use 0.45 for proteins in a wide range of molecular weights.
Product Size Qty Cat # 0.2um Cat# 0.45um
Hybond 30cm x 4m 1 84-895 83-646R
Rolls 15cm x 4m 1 - - - 85-549
8x9cm 25 84-958 85-554
Hybond 10x10cm 10 84-947 85-553
Sheets 14x16cm 25 - - - 85-551
20x20cm 10 84-922 - - -
Hybond 8x9cm 10 84-974 85-556
Sándwiches 14x16cm 10 - - - 85-555