•  Used to make polyacrylamide gels for protein electrophoresis and sequencing

IBI acrylamide powder can be used with IBI bisacrylamide powder to mix any concentration or ratio solution for making polyacrylamide gels. Polyacrylamide gels are used in protein electrophoresis and sequencing.

• Utilizado en la precipitación de ácidos nucleicos

El acetato de amonio es una sal comúnmente usada en la precipitación de ácidos nucleicos. La presencia de NH4OAc 2,5 M en la reacción de precipitación hace que los contaminantes unidos iónicamente se disocien de los ácidos nucleicos permitiendo la recuperación de muestras puras.

Para preparar una solución madre de acetato de amonio 10 M, disolver 385 g de acetato de amonio en 400 ml de agua destilada. Ajustar el volumen total a 500 ml con agua destilada. Esterilizar por filtración.

• Grado Molecular / Proteómica
•  Pureza >99% 

• For use in Tris-Borate electrophoresis buffer systems

Boric acid is an ingredient in the Tris-Borate buffer systems used in high voltage electrophoresis.

Boric acid powder can be used to make a concentrated stock solution of Tris-Boric Acid-EDTA (TBE) buffer.

• Se utiliza como agente de solidificación en la preparación de medios de cultivo microbiológicos

 El agar granulado es un extracto coloidal soluble en agua de ciertas especies de algas marinas rojas; Incluyendo Gelidium, Pterocladia y Gracilaria. Las impurezas, escombros, minerales y pigmentos se reducen a niveles específicos durante la fabricación. La mayoría de los microorganismos no pueden digerir el agar, lo que lo convierte en un excelente agente solidificante. El agar granulado también se puede utilizar para fines microbiológicos generales en los que la claridad no es un requisito estricto.

IBI Bacteriological Agar es ideal para su uso en investigación en microbiología y en aplicaciones de investigación en biología molecular que demuestren una excelente consistencia lote a lote y alta pureza. IBI Bac Agar es una excelente opción para su aplicación de investigación.

Guarde a temperatura ambiente, herméticamente cerrado y proteja de la humedad y la luz.

IBI Bacteriological Agar is available in multiple sizes:

IB49171 - 1kg
IB49172 - 2.5kg
IB49173 - 5kg
IB49174 - 10kg

• Se utiliza para el aislamiento, la identificación y el cultivo de hongos

El Agar Dextrose de Papa de IBI se utiliza para el aislamiento, la identificación y el cultivo de hongos. El agar de dextrosa de patata se utiliza también en el cultivo y aislamiento de levaduras y mohos de productos lácteos y alimenticios. Bajar el pH a 3,5 ± 0,1 inhibe el crecimiento bacteriano y ayuda en el aislamiento de hongos.

Guarde a temperatura ambiente (menos de 30 ° C), herméticamente cerrado y proteja de la humedad y la luz.

Triptona al 1,0%, Extracto de Levadura al 0,5%, Cloruro Sódico al 1,0%, Agar al 1,5%

• Contiene caseína digerida peptona, cloruro de sodio, extracto de levadura y agar
• Para mantener y propogating la cultura del coli

El Agar Miller LB de IBI se utiliza en estudios de genética molecular, así como en el mantenimiento y propagación de E. coli en procedimientos moleculares y de microbiología. La inclusión de caseína peptona y extracto de levadura proporciona factores de crecimiento esenciales para las bacterias; Tales como nitrógeno, azufre, minerales y vitaminas. El cloruro de sodio proporciona los electrolitos esenciales. El agar se usa como un agente de solidificación.

Para preparar, mezclar 40 g del medio en 1 L de agua purificada hasta que se dispersen uniformemente. Calentar con agitación repetida y hervir durante un minuto para disolver completamente. A continuación, distribuir y autoclave a 121,0 ° C durante 15 minutos.

Guarde a temperatura ambiente, herméticamente cerrado y proteja de la humedad y la luz.

• Specifications

• Tested and certified for optimal use in NGM
• Bacteriological grade for maximum efficiency
• Lot certified to ensure consistent results

HOJA TÉCNICA

Aplicaciones:

• Electroforesis de ácido nucleico (analítica y preparativa)
• Electroforesis de proteínas (incluída la inmunodifusión radial)
• Protocolos de borrado

Refinada mediante un proceso avanzado que excluye el uso de solventes orgánicos, La Agarosa LE produce un producto final más limpio con un impacto ambiental significativamente reducido. Puede usarse para análisis de proteínas y ácidos nucleicos de diversos tamaños (150pb a 6 kb). Elbajo EEO (<- 0.13 m) promueve una mayor movilidad electroforética, lo que proporciona una resolución mejorada y tiempos de ejecución más cortos. Esto también permite que macromoléculas y partículas más grandes (fragmentos subcelulares, virus, etc.) migren más libremente a través de la matriz de gel. El EEO consistentemente bajo también proporciona una reducción en la distorsión de banda (causada por contraflujo) que puede resultar de la presencia de iones negativos ricos en sulfato. Es fácilmente soluble, libre de nucleasas y fácil de usar. Es altamente transparente y exhibe una absorción excepcionalmente baja de agentes químicos de tinción.

APLICACIONES:

• Low Melt (LM) geles preparativos, en el procesamiento de gel.
• Mezcla 3: (e: 1): AFLP, STR, fragmentos de PCR de 50pb a 1 kb.
• Hi-Res (HR): STRs de AFLP, fragmentos de PCR de 20b a 800pb.

Características y Beneficios:

• Excelente Claridad: Mejora la visualización incluso a altas concentraciones.
• Geles Fuertes a Baja Concentración: Mejor manejo, menos roturas.
• Libre de ADNasas/ARNasas: Para todas las separaciones de Ácidos Nucleicos.

Descripción: 

Extremadamente pura, alto grado de Biología Molecular. En la agarosa de BIOLINE no se detecta actividad de ADNasas o ARNasas, forma geles fuertes. Debido a su bajo EEO el ADN tendrá una alta movilidad electroforética.

Catálogo    Presentación    Precio          EXISTENCIA

BIO-41025      500 grs.          SOLICITAR    3

BIO-41026      100 grs.          SOLICITAR     8

Certificada calidad ISO9001/cGMP

• Certified endotoxin-free and pyrogen-free
• 0.1 µm sterile-filtered into certified-clean polycarbonate bottles
• Double distilled and steam-sterilized
• Available in multiple sizes; sold individually and in multi-bottle cases
• Applications: nuclear transfer, transfection, transformation, cell and tissue culture, LAL testing, and all other aqueous solution preparations.

Descripción

IBI Cell Culture Grade Water is ideal for nuclear transfer, transfection, transformation, cell and tissue culture, LAL testing, and all other aqueous solution preparations that require stringent quality control.

IBI Cell Culture Grade Water is purified under stringent conditions. The purification process for this 18.2 megohm water includes double distillation, 0.1 µm sterile filtration, followed by steam sterilization in an autoclave. Product is also steam sterilized a second time in the final packaging. The water is certified endotoxin-free (to the maximum detection limit of ≤ 0.005 EU/mL by turbidimetric assay).

Our unique water purification process involves sterile-filtration through a 0.1 µm filter- the smallest filter (pore) size available- thus, removing all bacteria, viruses, and pyrogens. Sterility is further maintained by sterile-filtering directly into an aseptic polycarbonate bottle (or container). These FDA-approved polycarbonate bottles are non-leaching and allow for IBI water products to be autoclaved in its final packaging, ensuring terminal sterilization. Unlike PET bottles, IBI polycarbonate bottles can be autoclaved multiple times. If compromised sterility of an open product is a concern, our polycarbonate bottles allow for re-autoclaving to inactivate any contaminating nucleases.

Sterility of water is measured by the Sterility Assurance Level (SAL). IBI Cell Culture Grade Water maintains the highest level of sterility with a SAL=10-6. Thus, the chance of a living organism being contained in the water is one in a million. In order to maintain sterility, only open this product in a sterile environment, i.e. clean room, tissue culture fume hood, etc.

After manufacturing we test our water for up to three days. Each lot is individually assayed to ensure that it meets the stated specifications prior to being released from quality control. This is to ensure the levels of contaminates are within the accepted grades such as heavy metals at ≤ 0.00004%.

Catálogo                          Presentación                

IB42010                                     1 lt.                                                 

IB42011                                     6x1 lt.                                             

IB42012                                     12x1 lt.                                           

IB42020                                     2 lts.                                              

IB42021                                     6x2 lts.                                            

1.6% Triptona, 1.0% Extracto de Levadura, 0.5% Cloruro de Sodio

¡ Medio de crecimiento prepesado de alta calidad con pH preestablecido para mayor comodidad !

• For use in cell culture media to inhibit gram-positive and gram-negative bacteria

Is a semi-synthetic antibiotic which interferes with cell wall synthesis of gram negative bacteria by inactivating transpeptidases on the inner surface of the cell membrane. Carbenicillin displays low toxicity to plant tissues.

Is commonly used as a selective antibiotic for resistive Agrobacterium and E. coli at typical concentrations of 100 – 500 ug/ml in water.

A stock solution of antibiotics dissolved in H2O should be sterilized by filtration through a 0.22-micron filter.

Is packaged in a small glass amber bottle and shipped on blue ice packs

• Used in nucleic acid extraction procedures to remove phenol

Chloroform is a primary component used in nucleic acid extraction procedures to aid in the complete removal of phenol from the aqueous sample.

To use chloroform in extraction, mix chloroform and saturated phenol into a 1:1 solution. Mix with sample for ten seconds and spin down for three minutes. Remove aqueous layer and transfer into a new tube. Repeat until interface is no longer visible.

• Grado Molecular / Proteomica
• Pureza > 99%

Hoja Técnica

• For use in buffers and cell culture media

Sodium chloride is an ionic compound with the molecular formula of NaCl. Sodium chloride is the salt found most in the extracellular fluid of many multicellular organisms. It is also commonly used as the main component in saline solution.

This material is of non-animal and non-human origin.

• Molecular/Proteomic Grade 
• Purity > 99%

• Utilizado en la preparación enzimática para estabilizar la enzima

El ditiotreitol (DTT) se usa en preparaciones enzimáticas para prevenir la formación de disulfuros a partir de grupos sulfhidrilo. Esto estabiliza la enzima.

La DTT debe calentarse a temperatura ambiente antes de abrirla.

Las soluciones de DTT deben ser frescas antes de su uso. No esterilizar DTT o soluciones que contengan DTT.

• A reversible metalloprotease inhibitor useful for chelating metal ions.
• Molecular Weight: 372.24
• Storage Condition: ROOM TEMPERATURE
• White crystalline powder

• For use in biological and electrophoresis buffer systems
• A chelator of divalent cations
• For Research Use ONLY – NOT for Human Consumption

EDTA is commonly used in biological and electrophoresis buffer systems as a chelator of divalent cations. Some proteolytic enzymes and nucleases require divalent cations for activity. The addition of EDTA reduces the chance of sample degradation.

EDTA Disodium salt can be used to make a concentrated stock of buffer solutions.

This product is for Research Use ONLY and NOT for Human consumption.

• For use in biological and electrophoresis buffer systems

IBI 0.5M EDTA solution (pH 8.0) is ideal for biochemistry and molecular biology applications requiring a chelator of divalent metal ions.

Ethylenediamine tetraacetic acid solution can be used to elucidate the role of antigen retrieval in immunostaining of ethanol fixed smears.

For Research Use Only - Not For Human Consumption.

• 200 Proof Ethanol denatured with 5% methanol
• Used for precipitating nucleic acids

Ethanol is widely used for precipitating nucleic acids. The nucleic precipitate, which is formed in the presence of moderate concentrations of monovalent cations, is recovered by centrifugation and redissolved in an appropriate buffer at the desired concentration.

200 Proof Ethanol is denatured with methyl alcohol.

Ethanol is also known as ethyl alcohol, alcohol anhydrous, denatured alcohol.

• Apex Yeast Extract is the water soluble extract of selected autolyzed yeast cells
• Rich in vitamins, especially B-complexes, amino acids and other growth factors
• Used in many culture media formulations as an excellent growth source

• Preparado a partir de levadura autolizada
• Para uso en la preparación de medios de cultivo microbiológicos

El extracto de levadura de IBI se utiliza en la preparación de medios de cultivo microbiológicos. El extracto de levadura es la porción soluble en agua de la levadura autolizada. La autólisis se controla cuidadosamente para conservar las vitaminas del complejo B de origen natural. Es un estimulador excelente del crecimiento bacteriano y se utiliza en medios de cultivo en lugar de, o además de, extracto de carne de vaca.

Almacene el producto a temperatura ambiente, mantenga firmemente sellado y proteja de la humedad y la luz.

• Used in hybridization, electrophoresis applications, and to denature DNA and RNA

Spectral Grade Formamide is used as a denaturant in hybridization and is used in hybridization buffers made from standard recipes. Formamide separates the two strands of the double helix. Can also be used as a cryoprotectant and gel stabilizer. Ideal for preparing brain sections of mice for BrdU immunohistochemistry and FISH analysis of HeLa cells.

IBI Formamide is supplied in an amber glass bottle, it is distilled, deionized, and packaged under nitrogen to prevent oxidation. Bottles that have been opened and stored should be deionized prior to use by mixing 1 gm of mixed bed resin per 10 ml of formamide for 30 minutes. It is a clear liquid amide derived from formic acid.

The purity of IBI formamide is determined by way of gas chromatography.

• Used in preparation of polyacrylamide gels for electrophoresis
• For sample preparation to increase sample density.
• Used in sample preparation to prevent freezing.

IBI glycerol is ideal for both sample preparation and preparation of polyacrylamide gels for electrophoresis. A concentration measuring 5% - 10% glycerol increases the density of a sample so that the sample layers the bottom of the gel well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.

• Molecular/Proteomic Grade 

• Purity > 99%

HOJA TÉCNICA

• Purity > 99%
• Free of RNase, DNase, endonucleases & endotoxins
• Free of proteins and proteases
• Prepared & packaged under sterile, automated conditions in an ISO certified facility

• Molecular/Proteomic Grade: Purity > 99%
• Free of RNase, DNase, endonucleases & endotoxins
• Free of proteins and proteases
• Prepared & packaged under sterile, automated conditions in an ISO certified facility

SOLICITAR PRECIO

• 238.3 g/L HEPES
• Manufactured in an ISO 9001:2008/13485:2003 certified facility in the USA
• High quality raw material of USP or multi-compendial grade
• Lot-to-lot consistency for optimal cell culture performance
• Complete QA/QC release and testing

Description

HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulphonic acid) proveds an alternative to the use of bicarbonate for buffering because it does not require elevated levels of CO2. Unlike the low pKa of bicarbonate, HEPES has a pKa of 7.66 making it a much stronger buffer in the pH 7.2-7.6 range than bicarbonate buffer. Potential disadvantages of HEPES include toxicity over 100 mM and lack of nutritive value to the cell. The recommended working concentration is 10-25 mM. 

• Para uso en electroforesis de poliacrilamida y buffers de cultivo de células.

HEPES is a zwitterionic buffer ideally suited for cell culture in a range of 10 to 25 mM. HEPES has a buffering range of pH 6.8 to 8.2.

HEPES is also a component of Tris-HEPES buffer for polyacrylamide electrophoresis of proteins.

• For use in polyacrylamide electrophoresis buffer systems.
• Ideal cell culture buffer.
• Maintains physiological pH in cell culture applications.

HEPES is a zwitterionic biological buffer ideally suited for cell culture due to its ability to maintain physiological pH despite changes in carbon dioxide concentration. This property distinguishes HEPES from bicarbonate buffers which are also used in cell culture applications. Another characteristic of HEPES is that its disassociation decreases as temperature decreases making it a more effective buffer agent for maintaining enzyme structure and function at low temperatures.

Typical concentrations range from 10 to 25 mM. HEPES has a buffering range of pH 6.8 to 8.2.

HEPES is also a component of Tris-HEPES which is a buffer commonly used in polyacrylamide gel electrophoresis of proteins.

Offered in 50g, 250g, 500g and 1Kg sizes for your convenience.

• Molecular/Proteomic Grade: Purity > 99%
• Manufactured using ultra pure 4 fold purified water
• Free of RNase, DNase, endonucleases & endotoxins
• Free of proteins and proteases
• Prepared & packaged under sterile, automated conditions in an ISO certified facility

• Molecular/Proteomic Grade: Purity > 99%
• Manufactured using ultra pure 4 fold purified water
• Free of RNase, DNase, endonucleases & endotoxins
• Free of proteins and proteases
• Prepared & packaged under sterile, automated conditions in an ISO certified facility

• Ionic detergent that denatures most proteins and used to isolate RNA

Guanidine HCl is an ionic detergent that rapidly and effectively denatures most proteins. It is also used in the isolation of RNA.

• Ionic detergent that denatures most proteins and used to isolate RNA
• Contains Guanidine HCl (6 M) and EDTA (25 mM)

Guanidine HCl (Hydrochloride) Solution is an ionic detergent that rapidly and effectively denatures most proteins. It is also used in the isolation of RNA.

• Pureza: > 99%
• Free of RNase, DNase, endonucleases & endotoxins
• Free of proteins and proteases
• Prepared & packaged under sterile, automated conditions in an ISO certified facility

• High quality hydrolysate produced by enzymatic digestion of animal tissues
• Amino Nitrogen (%) 2.6-3.0
• Total Nitrogen (%) 12-15.55
• Ash (%) 4.7

Apex Peptone is a high quality hydrolysate produced by enzymatic digestion of animal tissues. It is widely used in culture media and has been used extensively in the manufacture of toxins, vaccines and other biological products. Typical analysis: Amino Nitrogen (%) 2.6-3.0, Total Nitrogen (%) 12-15.55, pH (2% solution) 6.5 – 7.5, Ash (%) 4.7. 

• Molecular/Proteomic Grade: Purity > 99%
• Free of RNase, DNase, endonucleases & endotoxins
• Free of proteins and proteases
• Prepared & packaged under sterile, automated conditions in an ISO certified facility

•  Ionic detergent used to denature proteins

SDS is an ionic detergent used to denature proteins in hybridization, nucleic acid purification, and electrophoresis buffer systems. SDS is also used to dissociate nucleic acid protein complexes in DNA extraction protocols, to disrupt cell membranes, and to prepare prehybridization and/or hybridization solutions.

To make a 10% SDS solution, dissolve 100 g of SDS in 900 ml of ddi water. Heat to 68⁰ C to assist dissolution. Adjust the pH to 7.2 by adding a few drops of HCl. Adjust the total volume to 1 L with ddi water, then dispense into aliquots.

• One dry pack makes 25 liters of 1X solution
• Ultra Pure Grade
• Ideal for DNA Recovery & In-gel Manipulations
• Low Ionic Strength
• Better Resolution of Large DNA Fragments: >12 Kb DNA

• Ideal for DNA Recovery & In-gel Manipulations
• Low Ionic Strength
• Better Resolution of Large DNA Fragments: >12 Kb DNA
• Convenient Packaging Options Minimize Weighing & Mixing
• More compatible with in-gel manipulations and band recovery procedures than TBE

Description

Apex™ BioResearch Products are manufactured using ultra pure materials under the industry's most stringent quality procedures and offered at a very competitive price without any loss to quality or reliability.

TAE is an extensively used buffer for agarose gel electrophoresis applications requiring high resolution and separation of high molecular weight, double-stranded DNA. TAE Buffer is more compatible with in-gel manipulations and band recovery procedures than TBE Buffer. A single strength (1X) solution contains 0.04 M Tris-Acetate and 0.001 M EDTA with a final pH of 8.0. 

• Molecular/Proteomic Grade: Purity > 99%
• Free of RNase, DNase, endonucleases & endotoxins
• Free of proteins and proteases
• Prepared & packaged under sterile, automated conditions in an ISO certified facility

• Molecular/Proteomic Grade: Purity > 99%
• Free of RNase, DNase, endonucleases & endotoxins
• Free of proteins and proteases
• Prepared & packaged under sterile, automated conditions in an ISO certified facility

• 1X: 0.089 M Tris - Borate, 0.002 M EDTA, pH 8.3
• Molecular/Proteomic Grade: Purity > 99%
• Free of RNase, DNase, endonucleases & endotoxins
• Free of proteins and proteases
• Prepared & packaged under sterile, automated conditions in an ISO certified facility

• 1X: 0.089 M Tris - Borate, 0.002 M EDTA, pH 8.3
• Molecular/Proteomic Grade: Purity > 99%
• Manufactured using ultra pure 4 fold purified water
• Free of RNase, DNase, endonucleases & endotoxins
• Free of proteins and proteases
• Prepared & packaged under sterile, automated conditions in an ISO certified facility

• Puede usarse convenientemente para reducciones en un ambiente de laboratorio más saludable
• Estable en soluciones ácidas y básicas (pH 1.5 a 9). También es estable al aire, no volátil y no reactivo
• Adecuado para aplicaciones de secuenciación de ADN de alto rendimiento

TCEP es un agente reductor soluble en agua, cristalino, puro, adecuado para la investigación de proteómica y genómica. Reduce los enlaces disulfuro intermoleculares e intra dentro de las proteínas.

TCEP HCl es una alternativa para el beta-mercaptoetanol (BME) / ditiotreitol (DTT). A diferencia de la TDT o la BME, el TCEP inodoro puede usarse convenientemente para reducciones en un ambiente de laboratorio más saludable. Es compatible con todos los tampones biológicos comunes utilizados para la purificación y conjugación de proteínas. (Por ejemplo, purificación de proteínas marcadas con 6 x His / GST). El TCEP reduce de forma selectiva y completa los enlaces disulfuro solubles en agua más estables en menos de 5 minutos incluso a una concentración muy baja (5 mM).

Apex brand Tegosept is widely used as a fly food preservative. Odorless, tasteless and non-toxic (sanctioned for food use), Apex Tegosept is active at low concentrations against a broad spectrum of microorganisms.

Synonyms: Benzoic acid, p-hydroxy-, methyl ester; methyl paraben; nipagen; Lexgard M. 

SOLICITAR PRECIO

HOJA TÉCNICA

• Un catalizador usado para fabricar geles de poliacrilamida para electroforesis y secuenciación de proteínas

TEMED (N, N, N ', N' - tetrametiletilén - diamina) se utiliza como catalizador para la polimerización de geles de poliacrilamida para electroforesis y secuenciación de proteínas.

• Specifications
• Certificate of Analysis
• SDS
• Technical Information

• Carbamimidoylazanium Thiocyanate
• Purity > 99%

HOJA TÉCNICA

• Apex Tryptone is a pancreatic digest of casein
• Contains all the amino acids found in casein as well as larger peptide fractions
• Excellent nutrient for use in culture media
• Amino Nitrogen (%) 3.9-4.2
• Total Nitrogen (%) 10-13.3
• Ash (%) 6.0

Apex Tryptone (Casein Peptone Type I) is a pancreatic digest of casein containing all the amino acids found in casein as well as larger peptide fractions. It is an excellent nutrient for use in culture media for producing antibiotics, toxins, enzymes and other biological products. This product is widely used in the pharmaceutical and veterinary industries and in diagnostic culture media. Typical Analysis: Amino Nitrogen (%) 3.9-4.2, Total Nitrogen (%) 10-13.3, pH (2% solution) 6.5 – 7.5, Ash (%) 6.0. 

• A pancreatic digest of casein
• Used as a nitrogen source in culture media

IBI’s Tryptone is a pancreatic digest of casein and used as a nitrogen source in culture media. It is capable of supporting growth of fastidious and non-fastidious microorganisms. Non-detectable concentration of carbohydrates allows its use in differentiating bacteria on the basis of their ability to ferment various carbohydrates. It can be used for indole production because of its high tryptopane concentration. It is also recommended as a casein peptone in media used for detecting bacteria in dairy products, drinking and/or waste water, and in antibiotic test media.

Store at room temperature, tightly sealed, and protect from moisture and light.

• Storage Condition: ROOM TEMPERATURE
• Reagent for stabilization of buffer formulations and electrophoresis of biological molecules. 

20-200          500 grs.

20-201            1 kg.

• Molecular/Proteomic Grade: Purity > 99%
• Free of RNase, DNase, endonucleases & endotoxins
• Free of proteins and proteases
• Prepared & packaged under sterile, automated conditions in an ISO certified facility

• 1X: 0.025 M Tris, 0.192 M Glycine, pH 8.3
• Molecular/Proteomic Grade: Purity > 99%
• Manufactured using ultra pure 4 fold purified water
• Free of RNase, DNase, endonucleases & endotoxins
• Free of proteins and proteases
• Prepared & packaged under sterile, automated conditions in an ISO certified facility

HOJA TÉCNICA

• Tris-Glycine 10X liquid concentrate. A 1X working solution contains 0.025M Tris base, 0.192 M Glycine.
• 1X buffer is used as transfer buffer for Western Blotting procedures.
• SDS can be included to a final concentration of 0.1% w/v to increase transfer efficiency from polyacrylamide gels that do not include SDS.
• Storage Condition: COLD
• Makes 40L of 1X solution

• Electrophoresis buffer solution containing Tris, Glycine, and SDS

Tris-Glycine SDS Buffer is used for polyacrylamide electrophoresis. It can be used in both native and denaturing (SDS) polyacrylamide gel electrophoresis of proteins. The versatility of Tris-Glycine Buffer enables the accurate separation of proteins in the range of 5 – 500 kDa.

With a Tris-Glycine-SDS buffer, the protein range for a 10% and 16% polyacrylamide gel is 20-250 kDa.

• 2-amino-2-hydroxymethyl-propane-1,3diol-hydrochloride
• Molecular/Proteomic Grade: Purity > 99%
• Free of RNase, DNase, endonucleases & endotoxins
• Free of proteins and proteases
• Prepared & packaged under sterile, automated conditions in an ISO certified facility

• For use in biological and electrophoresis buffer systems

Tris-HCl is commonly used in biological and electrophoresis buffer systems.

Tris-HCl can be used to make a concentrated stock of buffer solutions.

• Non-ionic detergent used to denature cell membranes and to break up protein aggregates

Triton X-100 is a non-ionic detergent used to denature cell membranes without denaturing the protein. Triton X-100 breaks up protein aggregates to promote enzymatic activity.

• Se utiliza como tinción de seguimiento para seguir el progreso de la separación de electroforesis
• Co-migra con moléculas de ADN a alrededor de 5Kb

Xileno Cyanol FF se utiliza como un colorante de seguimiento para supervisar el progreso de las separaciones de electroforesis. El colorante de seguimiento típicamente migra con las moléculas de ADN a alrededor de 5 Kb. Xileno Cyanol FF es el colorante de seguimiento de elección para monitorear el progreso de ciclos de electroforesis más largos.